The invention is a novel and efficient method to establish new embryonic stem (ES) cell lines from mammalian species and to maintain ES cells at the pluripotent state.
Stem Cell Research; Generation of ES and iPS cells, Propagation of adult stem cells, and Drug development.
The inventors predict that the methodology will provide a unique strategy to derive, maintain and propagate pluripotent ES and iPS cells across mammalian species.
This novel method is cost effective and more efficient to maintain pluripotent state of ES cells than the existing technologies. No need to use ES cell-competent serum, LIF and MEF. The method helps generate new stem cell lines from different mammalian species for stem cell banking. It could be possible to efficiently derive human iPS cells.