Novel Methods to Generate and Maintain Pluripotent Stem Cells

Track Code: 

The invention is a novel and efficient method to establish new embryonic stem (ES) cell lines from mammalian species and to maintain ES cells at the pluripotent state.

KU Medical Center (KUMC) researchers have discovered that addition of a novel compound prevents differentiation of mouse ES cells in culture in the absence of mouse embryonic fibroblast (MEF) and leukemia inhibitory factor (LIF). The compound also prevents differentiation of mouse ES cells in strong differentiating conditions. Data has shown that mouse ES cells cultured with this compound for several passages can generate chimeric mice. The researcher has also established new germ-line competent ES cell lines from mouse blastocyst using culture medium containing the compound in the absence of LIF or other inhibitors. In addition, the culture medium containing the compound facilitates derivation of induced pluripotent stem (iPS) cells from MEF and maintains undifferentiated feeder-free cultures of human ES cells. The inventors have recently derived new ES cell lines from RAT blastocyst using the compound-containing medium and are performing experiments to test for germ-line transmission. Learn more about the technology by downloading the published white paper.

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Stem Cell Research; Generation of ES and iPS cells, Propagation of adult stem cells, and Drug development.


The inventors predict that the methodology will provide a unique strategy to derive, maintain and propagate pluripotent ES and iPS cells across mammalian species.

Why it is better: 

This novel method is cost effective and more efficient to maintain pluripotent state of ES cells than the existing technologies. No need to use ES cell-competent serum, LIF and MEF. The method helps generate new stem cell lines from different mammalian species for stem cell banking. It could be possible to efficiently derive human iPS cells.

Licensing Associate: 
Aswini Betha, PhD · · 913-588-5713
Frank Schoenen
Jeffrey Aube
Soumen Paul
Debasree Dutta
Soma Ray
US 8,193,235